17 research outputs found
Evaluation of the frequency of the IL-28 polymorphism (rs8099917) in patients with chronic hepatitis C using zip nucleic acid probes, Kerman, Southeast of Iran
Get PDFPolymorphisms in the region of the interleukin IL-28 gene on chromosome 19 have been related with clearance of hepatitis C virus (HCV), a major human pathogen responsible for chronic hepatitis, cirrhosis and hepatocellular carcinoma. About 3 of the world's population is infected with HCV. The long-term response to therapy is influenced by many host and viral factors, and recent evidence has indicated that some host genetic polymorphisms related to IL-28 are the most powerful predictors of virological response in patients with HCV. This study assessed frequency of the IL-28 polymorphism (rs8099917) in 50 patients (39 men and 11 women ) with chronic hepatitis C using ZNA probe real time PCR new method . All patients were tested for genotype of HCV and the HCV viral load. In parallel, the levels of SGOT, SGPT and ALK enzymes were assessed. Treatment using Peg-interferon alpha with ribavirin was conducted for patients and subsequently samples were collected to detect any change in viral load or liver enzyme rates. The overall frequency of the TT allele is 74, TG allele 20 and GG allele 6 and the percent of patients who had T allele was 84. Clear reduction in viral load and liver enzymes was reported in patients with the T allele. Especially for genotype 1 which is relatively resistant to treatment, these alleles may have a role in this decline. In conclusion, we showed that IL-28 polymorphism rs8099917 strongly predicts virological response in HCV infection and that real-time PCR with Zip nucleic acid probes is a sensitive, specific and rapid detection method for detection of SNPs which will be essential for monitoring patients undergoing antiviral therapy
Study on viral nervouse necrosis (isolation, characterisation and pathogenesis) in golden grey mullet in the Caspian Sea and study of pathogenecity and possibility of transmission to the other fish species (Sturgeon fishes, Rutilus frisii kutum and reared Rainbow trout and Carp)
Get PDFStudy on Viral Nervouse necrosis (isolation, characterisation and pathogenesis) in Golden grey mullet in the Caspian Sea and study of pathogenecity and possibility of transmission to the other fish species (Sturgeon fishes, Rutilus frisii kutum and reared Rainbow trout and Carp) Viral Nervous Necrosis (VNN) is a worldwide disease affecting several species of cultured marine fish. For the past two decades, betanodavirus infections that cause Viral Nervous Necrosis (VNN) have emerged as major constraints on the culture and sea ranching of marine fish in almost all parts of the world. More than forty species mainly of marine origin have been so far affected and this number is likely to rise in future following the introduction of new species and the increase of aquaculture trade. Unknown acute mortality occurred in wild golden grey mullet Lisa auratus and Liza saliens in Iranian waters of Caspian Sea in recent years. In order to isolation and confirmation of causative agents of golden grey mullet mortality in the Caspian Sea, a complementary research investigation project was designed in 2005 and approved immediately in Iranian Fisheries Research Organization (IFRO). Many diagnostic aspects such as Virology (Cell culture and Elctereone Microscopy), Hemathology, Bacteriology, Histopathology, Molecular biology (NestedRT-PCR), Heavy metals measuerment and Serology (IFAT and IHC) were employed in mentioned multidiciplinary project. About 322 moribund fish samples which revealed skin darkening, erratic swimming behavior such as spiral and belly-up at rest and high distention of swimming bladder. Suspected samples were collected from coastal capture sites in iranian north proviences in 2006 till 2009. Targets tissue such as brain and eye were removed in strile condition and then kept in -80oC frezzer for cell culture and Nested-RT-PCR. Other tissue samples from liver, kidney, intestine, stomach, gill, skin and muscle, gall bladder and gonads were taken and fixed in 10% buffer formalin and same parts fixed in glutaraldehyde 3% for histopathology, IHC and EM respectively. Cytopathic effect (CPE) was observed in those cell cultures just six days after inoculation with the dilutions of the tested 312 homogenate supernatants. CPE in monolayers of cells cultured (SSN-1 cell line) was characterised by thin or rounded, refractile, granular cells with vacuoles. Nine samples were positive in virology assay. Nested- RT-PCR was done on suspected tissue samples and supernatant of CPE positive samples and 21 tissue samples and all CPE positive samples were positive. IFAT was selected as a confirmatory method for identifying viral strains replicating on cell cultures and carried out with rabbit anti-betanodavirus serum on suspected tissue samples and some smears of CPE positive samples. Some bright points approved betanodavirus antigen and confirmed cell culture and Nested-RT-PCR findings. In fixed tissue samples widespread and massive vacuolation were observed in brain, spinal cord, retina and optical nerve. In order to confirmation of diagnostic findings , IHC was done with monoclonal antibody antibetanodavirus and some red-brown points were observed. Theses findings revealed expected viral antigens and confirmed previous results. Moreover, virus particles with 25-30 nm in diameter were visualized in infected brain and retina using positive staining in TEM. Also pathogenicity test was employed to confirm the obtained results. So Guppy fish Poecilia reticulata and sturgeon fry were used instead of the experimental host due to ease of handling and susceptibility. After 15 days post infection, guppy bathed in VNN-infected tissue culture with 104 TCID50 showed clinical signs similar to naturally infected Golden grey mullet, and the mortality rate reached up to 100% in 75 dpi. When target organs were examined by cell culture isolation, serology, and histopathology, all revealed the presence of virus in the Guppy. Suspected supernatant injected to sturgeon fry through intravitreous injection and widespread vacuolation were observed in brain and spinal cord buy IHC and Real time PCR were negative. In conclusion, with attntion to obtained results in this investigation such as ecological factors, clinical signs, histopathological, virological and bacteriological results, molecular analysis, (IHC, IFAT, PCR), TEM demonstration, serological and hematological findings, it could be confirmed that VNNV was the main causative agent for disease outbreak in Golden grey mullet in Southern coastline of Caspian Sea
Explicit Solution of Non-Linear Fourth-Order Parabolic Equations via Homotopy Perturbation Method
No full textAssessment of modified variational iteration method in BVPs of high–order differential equations
No full textThis study has been devoted to investigate the semi-analytical solution of nonlinear differential equations with boundary value problems (BVPs). Modified variation iteration method has been utilized to solve some BVPs nonlinear differential equations. In this method, general lagrange multipliers have been introduced to construct correction functions for the problems. The multipliers can be identified optimally via the variational theory. The results have been compared with those of exact solutions and Homotopy Analysis Method (HAM). A clear conclusion can be drawn from the numerical results that proposed method provides excellent approximations to the solutions of this kind of problems in the terms of simplicity and accuracy, thus, it can be easily extended to other BVPs nonlinear differential equations and so can be found widely applicable in engineering sciences
Efficacy of mediterranean diet on blood biochemical factors in type II diabetic patients: A randomized controlled trial Tip II diyabetik hastalarda akdeniz diyetinin kan biyokimyasal faktörleri �zerindeki etkinli�i: Randomize kontrollü bir �alı�ma
No full textIntroduction: Diabetes mellitus is one of the most common endocrinologic disorder and the seventh cause of mortality worldwide. In Mediterranean dietary, consumption of plant source food such as vegetables, fruits, seeds, beans etc. is much emphasized. There are many studies showed that this regimen can decrease the risk of cardiovascular disease and factors associated in diabetes mellitus. Therefore, in this study we investigated the efficacy of this regimen on blood biochemical factors in diabetic patients referred to diabetes clinic in Gorgan. Methods: This was a clinical trial study in which 228 well-controlled type 2 diabetes mellitus patients between 40-60 years were included in the study. They were randomly assigned to two different groups: control and Mediterranean educational intervention group. They followed up for 6 months and before and after the intervention, we evaluated the glycemic and lipid profile and physiological parameters in them. Data were analyzed by SPSS software. Results: Totally 228 patients with diabetes mellitus type II between 40 to 60 years old were recruited. The mean age of patients was 57.3±9.28 years old. Majority of participants were female (77.2) with elementary level of literacy (77.6). The mean of fasting blood glucose was 192.50± 64.17 mg/dL in intervention group versus 208.48 ± 87.90 mg/dL in control group (P>0.05). This index was changed to 165.49± 50.39 mg/dL in intervention group which was significantly lower than the amount in control group (P<0.001). A clinically and statistically significant fall in HbA1c was observed in intervention group rather than control group (P<0.001). Conclusion: The present study represented a significant relationship between Mediterranean dietary intervention and both anthropometric and laboratory findings in patients with type 2 diabetes mellitus. We showed that this dietary could significantly lower the FBS, HbA1c, and LDL. Although we showed this meaningful difference, it should be more evaluated in Iranian race people to investigate more in this topic. © Copyright 2020 by Gazi University Medical Faculty
Variation of cutting forces in machining of f.c.c. single crystals
No full textThe final publication is available at Springer via http://dx.doi.org/10.1007/s00707-015-1418-zIn this study, micro-machining of f.c.c. single-crystal materials was investigated based on a hybrid modelling approach combining smoothed particle hydrodynamics and continuum finite element analysis. The numerical modelling was implemented in the commercial software ABAQUS/Explicit by employing a user-defined subroutine VUMAT for a crystal plasticity formulation to gain insight into the underlying mechanisms that drive a plastic response of materials in high deformation processes. The numerical studies demonstrate that cutting force variations in different cutting directions are similar for different f.c.c. crystals even though the magnitudes of the cutting forces are different
3D-printed biomimetic bone implant polymeric composite scaffolds
Get PDFopen access articleThis research introduced a new poly-ether-ether-ketone calcium hydroxyapatite (PEEK-cHAp) composite for a convenient, fast and inexpensive femur bone-implant scaffold with different lattice structures to mimic natural bone structure. Fused Deposition Modelling (FDM) was used to print a hybrid PEEK-based filament-bearing bioactive material suited for developing cHAp. Using FDM, the same bone scaffold PEEK will be fabricated, depending on the shape of the bone fracture. The scaffolds were examined for in-vitro bioactivity by immersing them in a simulated bodily fluid (SBF) solution. Furthermore, in-vitro cytotoxicity tests validated the suitability of the composite materials employed to create minimal toxicity of the scaffolds. After spreading PEEK nanoparticles in the grains, the suggested spherical nanoparticles cell expanded over time. The motif affected the microstructure of PEEK-cHAp in terms of grain size and 3D shape. The results established the proposed optimum design and suitable material for prospective bone implants, as required for biomimetic artificial bone regeneration and healing
New manifestations of electrophysiological remodeling of heart during experimental model of atrial fibrillation in cirrhotic rat isolated heart
No full textIntroduction: The present study is aimed to evaluate electrophysiological remodeling of atrioventricular (AV) node and ventricular conduction during experimental atrial fibrillation (AF) model in isolated heart of cirrhotic rats. Methods: Cirrhosis-induced electrophysiological remodeling was evaluated in 24 isolated retrogradely perfused rat hearts in 2 groups (control and cirrhotic). Cirrhosis was induced after 6 weeks of c ommon bile duct ligation in rats. Extracellular filed potential was recorded from upper atrium and right ventricle. The conduction time, refractoriness and frequency-dependent properties of AV node were characterized by specific stimulation protocols. Experimental AF was simulated by high-rate atrial pacing with random coupling intervals (range 75-125 ms). Results: Nodal conduction time and ventricular responsiveness were significantly increased in the cirrhotic rats compared to the control (95.8 ± 4.2 ms vs. 78.8 ± 3.3 ms) (P< 0.05). Nodal protective function during AF was potentiated with increased R-R interval, concealed beats, ventricular refractoriness and zone of concealment in the cirrhotic group. Cirrhosis evoked rate-dependent ventricular conduction time shortening with different patterns during arrhythmia. Conclusion: Cirrhosis-induced electrophysiological remodeling was shown by increased AV nodal conduction and shortened ventricular conduction. This electrophysiological remodeling may be considered as a new manifestation of cirrhotic cardiomyopathy in the heart, which can change ventricular rhythm during arrhythmia
